| 1. | Genetic group 3 included 2 new isolated strains and 3 representative strains ( usda6t , b15 , usda110 ) of b . japonicum Elkanii的参比菌株和9株待测葛藤根瘤菌组成,遗传群3由3株b |
| 2. | Other phenons did not grouped together with the representative strains . their exact taxonomic position required further study 而其它群未与已知分类地位的供试参比菌株聚在一起,其确切分类地位有待进一步研究。 |
| 3. | Phenon 5 included 4 new isolated strains and 3 representative strains ( usda6t , b15 , usda110 ) of b . japonicum . , the new isolated strains in this phenon probably are b . japonicum Japonicum的参比菌株聚在一起,群内相似性为83 ,初步判定这群葛藤根瘤菌为b japonicum 。 |
| 4. | In numerical taxonomy , phenon 1 included 9 new isolated strains and 1 representative strain ( usda761 ) of b . elkanii , the new isolated strains in this phenon probably are b . elkanii Elkanii的模式菌株( usda76 ~ t )聚在一起,群内相似性为80 ,初步判定群1供试葛藤根瘤菌为且belkanii 。 |
| 5. | Although belonging to the same genotype , representative strain jz1 was closed to usda110 , and ha1 was clustered into the same branch with usda110 , usda122 and usda127 , but wc7 and sd4 were clustered independently Jz1等供试菌和以usda110为代表的b . japonicum亲缘关系最近,序列差异小于1 ;代表菌ha1与usda110等同处于一个分枝中。 |
| 6. | Genetic group 7 included 4 new isolated strains and 3 representative strains ( ccbau100711 , ccbau10073 , ccbau10040 ) of b . yuanmingense . other genetic groups did not included representative strains . their genetic relatedness needs further study 而其它遗传群未与已知分类地位的供试参比菌株聚在一起,它们是不是新的遗传群,值得进一步研究。 |
| 7. | The 16s rdna / rna sequencing analysis of 4 morphological representative strains ( bd86 , yn78 , bd54 , shx9 ) was carried out . complete sequences of them were obtained and phylogenesis analysis was carried out of suborder cystobacterineae , their phylogenetic position was determined 挑选了4株形态较具代表性的菌株进行16srdna序列测定,以初步确定其归属,这4株菌编号分别为bd86 、 yn78 、 bd54 、 shx9 。 |
| 8. | Results of g + c mol % test shown that all slow - growing isolates were belong to the same species . more than 70 % dna - dna homologies were determined among 4 representative strains usda6 , and usda110 ( type strains of b . japonicum ) . low dna homologies were detected with usda76 ( type strain of b . elkanii ) 中l习花生根瘤菌遗传多样性和系统发育研究g + cmol %和dna一dna同源性分析结果表明,供试花生根瘤菌代表菌株的tm和g + cmol %均小于种内变异l隔度,表明供试花生根瘤菌均属j二一个种。 |
| 9. | The result of rflp analysis of pcr - amplified 16s rdna revealed that the rhizobia isolated from the root nodules of pueraria spp . had diversity at the level of species . the slow - growing rhizobia were divided into 7 groups on the similarity level of 90 % . group 1 included 4 new isolated strains and 2 representative strains of b . japonicum . group 2 included 1 type strain of b . elkanii and 3 new isolated strains 16srdnapcr rflp分析结果也表明葛藤根瘤菌具有种水平多样性,在90的相似性水平上,聚类图把慢生根瘤菌分成7群,群1与bjponicum的模式菌株聚在一起,群二与belhanii的模式菌株聚在一起,而其它群是独立的系统发育分支。 |